In vivo Spectral Analysis of Bladder Cancer Using Fourier Transform Infrared Spectroscopy , A comparative Study

Recently, spectroscopic analysis of cancer tissues has received considerable attention to be a diagnosis technique due to its sensitivity to biochemical variations in the samples. In this study Fourier Transform Infrared Spectroscopy (FTIR) was used to analyze, spectroscopicaly, a number of bladder cancer tissues compared with normal bladder tissues. Twenty samples were collected from ten patients, diagnosed with bladder cancer, from different hospitals without any pretreatment. From each patient two samples were collected, one of them was normal and the second was cancerous tissue. FTIR spectrometer was used to differentiate between normal and cancerous bladder tissues via the change in the spectra of these samples. The investigations detected obvious spectroscopic changes in the proteins (1650, 1550 cm), lipids (2925, 2850 cm) and nucleic acids (1080, 1236 cm). The results showed that FTIR spectroscopy holds much promising as a rapid, accurate, nondestructive method, and easy to use as an alternative method for identification and diagnosis of bladder cancerous tissues.